期刊
BIORESOURCE TECHNOLOGY
卷 133, 期 -, 页码 82-86出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.01.117
关键词
Cordycepin; Enzymatic acylation; Regioselectivity; 2-Methyltetrahydrofuran
资金
- Ph.D. Programs Foundation of Ministry of Education of China [200803071026]
- Natural Science Foundation of China [31071495]
- Fundamental Research Funds for the Central Universities [KYZ201002]
- PAPD
A total of nine lipases and three proteases were tested for enzymatic regioselective acylation(s) of cordycepin with vinyl acetate in organic media. The highest conversion with better initial reaction rate was achieved with immobilized Candida antarctica lipase B (Novozym 435). An eco-friendly solvent 2-methyltetrahydrofuran (MeTHF) was thought to be the most suitable reaction medium. Novozym 435 was found to be a useful biocatalyst for the 25-g scale syntheses of cordycepin acetate (96.2% isolated yield), and the biocatalyst displayed excellent regioselectivity and high operational stability during the transformation. The 5'-substituted cordycepin derivative was the sole detectable product from each acylation reaction. Novozym 435 could be recycled for the synthesis of cordycepin derivative on a 25-g scale and 63% of its original activity was maintained after being reused for 7 batches. MeTHF could be considered as an eco-friendly solvent for the large scale use in biotransformation. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据