期刊
BIORESOURCE TECHNOLOGY
卷 132, 期 -, 页码 178-181出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.12.075
关键词
Deep-sea fungus; Penicillium griseofulvum; Extracellular polysaccharide; Preparation; Characterization
资金
- Science and Technology Development Program of Shandong Province, China [2010GHY10509]
- National Oceanographic Center of Qingdao of China
- National Natural Science Foundation of China [41076086]
The deep-sea fungus Penicillium griseofulvum produces an extracellular polysaccharide, Ps1-1, when grown in potato dextrose-agar medium. Ps1-1 was isolated from the fermented broth using ethanol precipitation, anion-exchange and size-exclusion chromatography. Ps1-1 is a galactomannan with a molecular weight of about 20 kDa, and a molar ratio of mannose and glucose of 1.1:1.0. On the basis of one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) and mass spectroscopic analyses, Ps1-1 is composed of a long chain of galactofuranan and a mannose core. The galactofuranan chain consists of (1 -> 5)-linked beta-galactofuranose, with additional branches at C-6 consisting of (1 ->)-linked beta-galactofuranose residues and phosphate esters. The mannan core is composed of (1 -> 6)-linked alpha-mannopyranose substituted at C-2 by (1 ->)-linked alpha-mannopyranose residues, disaccharide and trisaccharide units of (1 -> 2)-linked alpha-mannopyranose. The investigation demonstrated that Ps1-1 was a galactofuranose-containing galactomannan differing from previously described extracellular polysaccharides. (C) 2012 Elsevier Ltd. All rights reserved.
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