期刊
BIORESOURCE TECHNOLOGY
卷 116, 期 -, 页码 147-154出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.04.018
关键词
Bioconversion; White rot; Deconstruction; Wood; Biodegradation
资金
- U.S. Department of Energy (DOE) Biomass Initiative Grant [GO18088]
Brown rot fungi Gloeophyllum trabeum and Postia placenta were used to degrade aspen, spruce, or corn stover over 16 weeks. Decayed residues were saccharified using commercial cellulases or brown rot fungal extracts, loaded at equal but low endoglucanase titers. Saccharification was then repeated for high-yield samples using full strength commercial cellulases. Overall, brown rot pretreatments enhanced yields up to threefold when using either cellulase preparation. In the best case, aspen degraded 2 weeks by G. trabeum yielded 72% glucose-from-cellulose, a 51% yield relative to original glucan. A follow-up trial with more frequent harvests showed similar patterns and demonstrated interplay between tissue modifications and saccharification. Hemicellulose and vanillic acid (G6) or vanillin (G4) lignin residues were good predictors of saccharification potential, the latter notable given lignin's potential active role in brown rot. Results show basic relationships over a brown rot time course and lend targets for controlling an applied bioconversion process. (C) 2012 Elsevier Ltd. All rights reserved.
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