期刊
BIORESOURCE TECHNOLOGY
卷 115, 期 -, 页码 117-125出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.01.054
关键词
Directed evolution; Biocatalysis; Enzyme engineering; High-throughput screening
资金
- National Institutes of Health [GM077596]
- National Academies Keck Futures Initiative on Synthetic Biology
- Biotechnology Research and Development Consortium (BRDC) [2-4-121]
- BP Energy Biosciences Institute
- National Science Foundation as part of the Center for Enabling New Technologies through Catalysis (CENTC) [CHE-0650456]
- National Research Foundation of Korea (NRF) [220-2009-1-D00033]
- National Research Foundation of Korea [220-2009-1-D00033] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Biocatalysis has emerged as a great addition to traditional chemical processes for production of bulk chemicals and pharmaceuticals. To overcome the limitations of naturally occurring enzymes, directed evolution has become the most important tool for improving critical traits of biocatalysts such as thermostability, activity, selectivity, and tolerance towards organic solvents for industrial applications. Recent advances in mutant library creation and high-throughput screening have greatly facilitated the engineering of novel and improved biocatalysts. This review provides an update of the recent developments in the use of directed evolution to engineer biocatalysts for practical applications. (C) 2012 Elsevier Ltd. All rights reserved.
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