期刊
BIORESOURCE TECHNOLOGY
卷 109, 期 -, 页码 271-276出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.11.078
关键词
Alkadiene; Botryococcene; Botryococcus braunii; Gene expression; Hydrocarbon
资金
- Core Research for Evolutionary Science and Technology of Japan Science and Technology Agency
To clarify the oil biosynthetic routes of the oil-producing green alga Botryococcus braunii, here the race-specific gene expression patterns were examined using representative strains of race A and race B producing fatty acid- and triterpene-derived hydrocarbon oils, respectively. The strain-specific gene expression patterns in the BOT-88-2 strain (race A) and the BOT-22 strain (race B) were revealed by transcriptome comparison and real-time PCR quantification. For race A, it was inferred from the gene expression patterns that the fatty acid elongation in the acyl-carrier-protein (acp)-bound form followed by further elongation in the coenzyme A (CoA)-bound form is the major route of oil biosynthesis. The fatty acids may be desaturated in both acp- and CoA-bound forms and once metabolized into glycerolipids prior to further elongation. For race B, relatively direct entry of photosynthetic products from the reductive pentose phosphate cycle into the mevalonate-independent triterpene biosynthesis was implicated. (C) 2011 Elsevier Ltd. All rights reserved.
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