期刊
BIORESOURCE TECHNOLOGY
卷 114, 期 -, 页码 365-369出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.03.076
关键词
Fungal pretreatment; Phanerochaete chrysosporium; Saccharification; Hydrogen production
资金
- National Natural Science Foundation of China [51178140, 30870037, 31100095]
- National Creative Research Groups [50821002]
- National Science & Technology Pillar Program [2008BADC4B01]
- China Postdoctoral Science Foundation [20110491053]
- State Key Laboratory of Urban Water Resource and Environment
- Harbin Institute of Technology [HC201114]
- Fundamental Research Funds for the Central Universities [HIT. NSRIF. 2011019]
The feasibility of fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enzymatic saccharification and H-2 production was investigated in this study. Firstly, cornstalk was pretreated with P. chtysosporium at 29 degrees C under static condition for 15 d, lignin reduction was up to 34.3% with holocellulose loss less than 10%. Microscopic structure observation combined FTIR analysis further demonstrated that the lignin and crystallinity were decreased. Subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from Trichoderma viride to produce fermentable sugars which were then fermented to bio-H-2 using Thermoanaerobacterium thermosaccharolyticum W16. The maximum enzymatic saccharification was found to be 47.3% which was 20.3% higher than the control without pretreatment. Upon fermentation of enzymatic hydrolysate, the yield of H-2 was calculated to be 80.3 ml/g-pretreated cornstalk. The present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-H-2 integrate with biological pretreatment and enzymatic saccharification. (C) 2012 Elsevier Ltd. All rights reserved.
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