期刊
BIORESOURCE TECHNOLOGY
卷 123, 期 -, 页码 279-283出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.07.057
关键词
Microalgae; Lipid production; Chlorella vulgaris; Two-stage process; Nitrogen limitation
资金
- Marine Biotechnology Program
- Ministry of Land, Transport and Maritime Affairs of Korea
- Ministry of Education, Korea
- Korea Institute of Marine Science & Technology Promotion (KIMST) [20090267] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A two-stage process, composed of growth under nutrient-rich conditions followed by cultivation under nitrogen starvation and controlled conditions of phosphate, light intensity, aeration, and carbon sources was applied for lipid production by the green alga Chlorella vulgar's. Using conditions without addition of nitrogen, 2 mg/L PO4-P, light intensity of 100 mu mol/m(2)/s and 0.25 vvm of air, about 43% of dry cell weight accumulated as lipids after 12 h, which equates to a lipid productivity of 77.8 mg/L/d. In a medium containing 5 mg/L NO3-N and 2 mg/L PO4-P, and at a light intensity of 100 mu mol/m(2)/s and 0.25 vvm of 2% CO2, about 53% of dry cell weight consisted of lipids after 24 h, representing a lipid productivity of 77.1 mg/L/d. The low amount of nutrients, moderate aeration and light intensity were helpful for increasing lipid productivity. (C) 2012 Elsevier Ltd. All rights reserved.
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