期刊
BIORESOURCE TECHNOLOGY
卷 119, 期 -, 页码 94-98出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.05.108
关键词
Acetoin; 2,3-Butanediol; Serratia marcescens; Cofactor engineering; Fermentation
资金
- Chinese National Program for High Technology and Development (863 Program) [2006AA02Z243]
- Shanghai Leading Academic Discipline Project [B505]
- State Key Laboratory Special Fund [2060204]
Cofactor engineering was employed to enhance production of acetoin by Serratia marcescens H32. 2,3-Butanediol was a major byproduct of acetoin fermentation by S. marcescens H32. In order to decrease 2,3-butanediol formation and achieve a high efficiency of acetoin production, nox gene encoding a water-forming NADH oxidase from Lactobacillus brevis was expressed. Batch fermentations suggested the expression of the NADH oxidase could increase the intracellular NAD(+) concentration (1.5-fold) and NAD(+)/NADH ratio (2.9-fold). Meanwhile, 2,3-butanediol was significantly decreased (52%), and the accumulation of acetoin was enhanced (33%) accordingly. By fed-batch culture of the engineered strain, the final acetoin titer up to 75.2 g/l with the productivity of 1.88 g/(1h) was obtained. To the best of our knowledge, these results were new records on acetoin fermentation ever reported. (C) 2012 Elsevier Ltd. All rights reserved.
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