期刊
BIORESOURCE TECHNOLOGY
卷 123, 期 -, 页码 184-188出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.07.055
关键词
Hydrogen production; Co-culture; Chlamydomonas reinhardtii; Bradyrhizobium japonicum
资金
- Key Project of Science and Technology Commission of Shanghai [09160500400]
- CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences
- SOA Key Laboratory for Polar Science, Polar Research Institute of China [KP201107]
- Shanghai Municipal Education Commission [J50401]
- Shanghai Normal University [DZL808]
- [NSCF31140070]
Co-cultivation of Bradyrhizobium japonicum with Chlamydomonas reinhardtii strain cc849 or the transgenic strain lba, which was hetero-expressed the gene of the soybean leghemoglobin apoprotein Lba in chloroplasts of the strain cc849, in Tris-acetate-phosphate (TAP) or TAP-sulfur free media, improved H-2 yield. H-2 production was 14 times and growth was 26% higher when strain lba and B. japonicum were co-cultured, as compared with cultivation of the algal strain alone under the same conditions. The increase in respiration rate or fast O-2 consumption by about 8 times in the co-cultures was the major reason for the improvement. (C) 2012 Elsevier Ltd. All rights reserved.
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