期刊
BIORESOURCE TECHNOLOGY
卷 124, 期 -, 页码 504-507出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.08.104
关键词
Xylose-fermentation; Tolerance; Industral Saccharomyces cerevisiae; LSM protein; Acetic acid
资金
- National High Technology Research and Development Program of China [2007AA05Z401]
- National Science & Technology Pillar Program of China [2007BAD66B02]
A current challenge of the cellulosic ethanol industry is to improve the resistance of inhibitors present in biomass hydrolysates. RNA-binding protein gene Ism6 was cloned from industrial Saccharomyces cerevisiae ZU-E8, which is able to conferment glucose and xylose, and transformed into ZU-E8 via expression vector pRS426. The positive transformant ZU-910 with over-expressing Ism6 was identified on the culture plates using high concentration of acetate and re-screened by fermentation test. Fermentation by the recombinants was performed in a medium containing 80 g/L xylose and 2 g/L acetic acid or 20 g/L NH4Ac/NaAc. After 96 h shaking-flask fermentation, ZU-910 utilized 90.2% xylose with an ethanol yield of 26.9 g/L, which was 8.5- and 10-fold higher than ZU-E8. Further, in the corn stover hemicellulosic hydrolysate fermentation, both the xylose conversion and ethanol production by ZU-910 was larger by 50% and 40% than ZU-E8. ZU-910 has also enhanced tolerance against furfural and SO42-. (C) 2012 Elsevier Ltd. All rights reserved.
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