期刊
BIORESOURCE TECHNOLOGY
卷 126, 期 -, 页码 80-86出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.09.055
关键词
Bacillus vallismortis fmb-103; Spore laccase; Degradation; Triphenylmethane dyes
资金
- National Natural Science Foundation of China [31201423, 31071605]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Youth Science and Technology Innovation Fund of Nanjing Agriculture University [Y201069]
The characterization of a spore laccase from Bacillus vallismortis fmb-103, isolated from textile industry disposal sites, is described. The activity was 6.5 U/g of dry spore with ABTS as the substrate. The enzyme was quite stable at high temperature. It retained more than 90% of its initial activity after 10 h at 70 degrees C. The enzyme demonstrated broad pH stability in both acidic and alkaline conditions. There was almost no activity loss at pH 3 over an extended period of time, and the relative activity remained at 82% and 38% at pH 7 and pH 9 after 10 days. NaN3, SDS, L-cysterine, Dithiothreitol, EDTA and NaCl inhibit the enzyme activity. Triphenylmethane dyes, including malachite green, brilliant green and aniline blue were efficiently degraded by the enzyme after 24 h in combination with a mediator with efficiencies of 76.84%, 96.56% and 81.17%, respectively. The reusability of spore laccase for decolorization dyes was also examined. (C) 2012 Elsevier Ltd. All rights reserved.
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