期刊
BIORESOURCE TECHNOLOGY
卷 117, 期 -, 页码 186-192出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.04.085
关键词
Laccase; Delignification; TMAH-GC-MS; Enzymatic hydrolysis; Ensilage
资金
- USDA-DOE Biomass Research and Development Initiative [68-3A75-4-137]
- Pennsylvania Agricultural Experiment Station
The aim of this study was to explore the synergies of laccase, a ligninolytic enzyme, with cellulose and hemicellulase amendments on ensiled corn stover. Molecular signals of lignin decomposition were observed by tetramethylammonium hydroxide thermochemolysis and gas chromatography-mass spectroscopy (TMAH-GC-MS) analysis. The significant findings suggest that ensilage might provide a platform for biological pretreatment. By partially hydrolyzing cellulose and hemicellulose into soluble sugars, ensilage facilitates laccase penetration into the lignocellulose complex to enhance lignin degradation. Downstream cellulose hydrolysis was improved 7% with increasing laccase loading rate. These results demonstrate the potential of enzymes, either directly amended or expressed by microbes during ensilage, to maximize utilization of corn stover for cellulosic biofuels and other downstream fermentations. (C) 2012 Published by Elsevier Ltd.
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