期刊
BIORESOURCE TECHNOLOGY
卷 104, 期 -, 页码 410-416出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.10.099
关键词
beta-Glucosidase; Cellobiose; Cellulosic ethanol; Corncobs; Simultaneous saccharification and fermentation
资金
- National Research Initiative [2006-35504-17359]
This study reports a new yeast strain of Clavispora NRRL Y-50464 that is able to utilize cellobiose as sole source of carbon and produce sufficient native beta-glucosidase enzyme activity for cellulosic ethanol production using SSF. In addition, this yeast is tolerant to the major inhibitors derived from lignocellulosic biomass pre-treatment such as 2-furaldehyde (furfural) and 5-(hydroxymethyl)-2-furaldehyde (HMF), and converted furfural into furan methanol in less than 12 h and HMF into furan-2,5-dimethanol within 24 h in the presence of 15 mM each of furfural and HMF. Using xylose-extracted corncob residue as cellulosic feedstock, an ethanol production of 23 g/l was obtained using 25% solids loading at 37 degrees C by SSF without addition of exogenous beta-glucosidase. Development of this yeast aids renewable biofuels development efforts for economic consolidated SSF bio-processing. Published by Elsevier Ltd.
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