期刊
BIORESOURCE TECHNOLOGY
卷 104, 期 -, 页码 298-304出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.11.080
关键词
Laccase; Lignocellulose; Detoxification; Folin-Ciocalteu assay; Headspace-SPME/GC-MS
资金
- Wacker Chemie AG
- Federal Ministry of Education and Research, Germany [FKZ: 0315193]
- Deutsche Forschungsgemeinschaft DFG [DFG-GZ: INST 95/1061-1 FUGG]
The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5 U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24 h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1 day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions. (C) 2011 Elsevier Ltd. All rights reserved.
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