期刊
BIORESOURCE TECHNOLOGY
卷 119, 期 -, 页码 373-383出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.05.136
关键词
Municipal solid waste anaerobic reactor; 16S rDNA clone library; Real-time PCR; DGGE; FISH/CARD-FISH
资金
- Methanum Environmental and Energy Solutions
- Financiadora de Estudos e Projetos (FINEP)
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Pro-reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq-UFMG)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
The prokaryotic diversity of an anaerobic reactor for the treatment of municipal solid waste was investigated over the course of 2 years with the use of 16S rDNA-targeted molecular approaches. The fermentative Bacteroidetes and Firmicutes predominated, and Proteobacteria, Actinobacteria, Tenericutes and the candidate division WWE1 were also identified. Methane production was dominated by the hydrogenotrophic Methanomicrobiales (Methanoculleus sp.) and their syntrophic association with acetate-utilizing and propionate-oxidizing bacteria. qPCR demonstrated the predominance of the hydrogenotrophic over aceticlastic Methanosarcinaceae (Methanosarcina sp. and Methanimicrococcus sp.), and Methanosaetaceae (Methanosaeta sp.) were measured in low numbers in the reactor. According to the FISH and CARD-FISH analyses, Bacteria and Archaea accounted for 85% and 15% of the cells, respectively. Different cell counts for these domains were obtained by qPCR versus FISH analyses. The use of several molecular tools increases our knowledge of the prokaryotic community dynamics from start-up to steady-state conditions in a full-scale MSW reactor. (C) 2012 Elsevier Ltd. All rights reserved.
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