期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 11, 页码 6479-6486出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.03.022
关键词
Biomass; Alfalfa; Bioethanol; Pretreatment; Lignin-modified transgenics
资金
- Forage Genetics International
Alfalfa (Medicago sativa L) biomass was evaluated for biochemical conversion into ethanol using dilute-acid and ammonia pretreatments. The two alfalfa lines compared were a reduced S-lignin transgenic cultivar generated through down regulation of the caffeic acid O-methyltransferase gene and a wild-type control. Both were harvested at two maturities. All the samples had similar carbohydrate contents including a mean composition of 316 g glucan and 497 g total neutral carbohydrates per kg dry biomass, which corresponds to a theoretic ethanol yield of 3821/ton. Ethanol yields for alfalfa stems pretreated with dilute-acid were significantly impacted by harvest maturity and lignin composition, whereas when pretreated with dilute-ammonia, yield was solely affected by lignin composition. Use of a recombinant xylose-fermenting Saccharomyces strain, for converting the ammonia pretreated alfalfa samples, further increased ethanol yields. Ethanol yields for the xylose-fermenting yeast were 232-278 l/ton and were significantly enhanced for the reduced S lignin cultivars. Published by Elsevier Ltd.
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