期刊
PEPTIDE SCIENCE
卷 110, 期 5, 页码 -出版社
WILEY
DOI: 10.1002/bip.23081
关键词
biocompatible surfaces; cell adhesion; diagnostic device; inflammation; integrin alpha 4 beta 1; peptidomimetic
资金
- CHIESI Foundation, Parma, Italy
- Axa Research Fund
Persistent accumulation of immune cells mediated by alpha 4 beta 1 integrin (VLA-4) is a hallmark of the inflammatory diseases and of chronic inflammation observed in the affected tissues of autoimmune diseases. Aiming at exploring new methods for monitoring the course of the inflammatory processes, we designed the first peptide-functionalized nanostructured devices capable to mimic the high-density multivalency binding between the alpha 4 beta 1 integrin-expressing cells and the ligands overexpressed on the endothelial surfaces, in the proximity of the sites of inflammation. Specifically, we describe the first examples of monolayers constituted by dye-loaded zeolite L crystals, coated with alpha 4 beta 1 integrin peptide ligands, and we analyze the adhesion of model Jurkat cells in comparison to non-alpha 4 beta 1 integrin-expressing cells. In particular, the peptidomimetic diphenylurea-Leu-AspVal- diamine allows significant and selective detection of alpha 4 beta 1 integrin-expressing Jurkat cells, after very rapid incubation time, supporting the possible implementation in a diagnostic device capable to detect the desired cells from biological fluids, obtainable from patients in a noninvasive way.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据