期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 18, 页码 8357-8367出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.04.054
关键词
Biohydrogen; Metabolic engineering; Biophotolysis; Photofermentation; Dark fermentation
资金
- Korea Institute of Energy Research
- National Research Foundation
- Korean Government (MEST) [NRF-2009-C1AAA001-20090093214]
- Brain Korea 21 program
- Pusan National University
- National Research Council of Science & Technology (NST), Republic of Korea [KIER-B12405] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The improvement of H-2 production capabilities of hydrogen (H-2)-producing microorganisms is a challenging issue. Microorganisms have evolved for fast growth and substrate utilization rather than H-2 production. To develop good H-2-producing biocatalysts, many studies have focused on the redirection and/or reconstruction of cellular metabolisms. These studies included the elimination of enzymes and carbon pathways interfering or competing with H-2 production, the incorporation of non-native metabolic pathways leading to H-2 production, the utilization of various carbon substrates, the rectification of H-2-producting enzymes (nitrogenase and hydrogenase) and photophosphorylation systems, and in silico pathway flux analysis, among others. Owing to these studies, significant improvements in the yield and rate of H-2 production, and in the stability of H-2 production activity, were reached. This review presents and discusses the recent developments in biohydrogen production, with a focus on metabolic pathway engineering. (C) 2011 Elsevier Ltd. All rights reserved.
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