期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 20, 页码 9605-9612出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.07.034
关键词
Lignocelluloses; Tween 20; Cellulase; Saccharification
资金
- JST/JICA, SATREPS, Sustainable Integration of Local Agriculture and Biomass Industries
In this work, we found that Tween 20 treatment (0-8 mM) contributed to the cell wall collapse of most samples except for those with high lignin contents and high crystallinity. Cell wall collapse contributed to the formation of 10- to 50-nm pores and not only increased the monolayer saturation amount of adsorbed cellulase about 3-3.6 times but also increased the cellulase adsorption rate (D-e/r(2)) about 160-880 times. Moreover, cellulose conversion at 72 h was also increased 8.7-21.5% by Tween 20 treatment. On the other hand, the adsorption of Tween 20 on Avicel (microcrystalline cellulose) hindered the cellulase reaction (adsorption and saccharification). The effect of Tween 20 treatment on the crystalline part was insignificant for both lignocelluloses and Avicel. It was found that some degree of pretreatment (e.g. lignin removal) that enhances Tween 20 diffusion into samples is necessary to obtain the structural effects of Tween 20. (C) 2011 Elsevier Ltd. All rights reserved.
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