期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 10, 页码 6295-6298出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.02.016
关键词
BSA; Enzymatic hydrolysis; Continuous hydrolysis; Enzyme stability; Cellulose
资金
- National Research Initiative of the USDA Cooperative State Research, Education and Extension Service [2004-35504-14668]
- National Institute of Standards and Technology [60NANB1D0064]
- Chair in Environmental Engineering at Center for Environmental Research and Technology of the Bourns College of Engineering at UCR
Bovine serum albumin (BSA) was applied as a model non-catalytic protein to enzymatic hydrolysis of Avicel and dilute acid pretreated corn stover at different reaction conditions to improve the understanding of its ability to enhance cellulose hydrolysis. Addition of BSA improved the 72 h hydrolysis yields in shake flasks by up to 26% for both substrates by reducing de-activation of the exoglucanases and by facilitating reductions in particle size and crystallinity during a magnetically stirred pre-incubation step. The enzyme stabilizing effect of BSA addition was most striking for batch hydrolysis in a stirred tank reactor, with glucose yields increasing by 76% after 72 h for Avicel and by 40% after 145 h for corn stover. Application of BSA to continuous hydrolysis for a mean residence time of 24 h gave 33% and 40% higher glucose yields for corn stover and Avicel compared to the controls. (C) 2011 Elsevier Ltd. All rights reserved.
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