期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 10, 页码 6159-6166出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.03.025
关键词
Medium-chain-length; polyhydroxyalkanoates; Waste activated sludge; Mixed culture; Pseudomonas aeruginosa
资金
- Korea National Research Foundation [2010-0022533]
- Brain Korea 21 Projects
- National Research Foundation of Korea [2010-0022533] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The potential use of activated sludge for the production of medium-chain-length polyhydroxyalkanoates (MCL-PHAs) was investigated. The enrichment of bacterial populations capable of producing MCL-PHAs was achieved by periodic feeding with nonanoic acid in a sequencing batch reactor (SBR). Denaturing gradient gel electrophoresis analysis revealed Pseudomonas aeruginosa strains to be predominant in the bacterial community during the SBR process. The composition of PHA synthesized by the enriched biomass from nonanoic acid consisted of a large concentration (>89 mol%) of MCL monomer units and a small amount of short-chain-length monomer units. Under fed-batch fermentation with continuous feeding of nonanoic acid at a flow rate of 0.225 g/L/h and a C/N ratio of 40, a maximum PHA content of 48.6% dry cell weight and a conversion yield (Y(p/s)) of 0.94 g/g were achieved. These results indicate that MCL-PHA production by activated sludge is a promising alternative to typical pure culture approaches. (C) 2011 Elsevier Ltd. All rights reserved.
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