期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 3, 页码 3595-3598出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.10.073
关键词
Bacillus subtilis CGMCC 2108; Poly-(gamma-glutamic acid); Viscosity; Enzyme activity; CaCl2
CaCl2 was used as a novel additive to enhance poly-(gamma-glutamic acid) (7-PGA) production by Bacillus subtilis strain CGMCC 2108. Addition of CaCl2 to medium effectively reduced viscosity of culture broth, and increased consumption of extracellular glutamate by 11.4%, leading to gamma-PGA yield of 9.07 g/l, compared to 7.138 g/l in control. CaCl2 also increased activities of three key enzymes around the crucial 2-oxoglutarate branch of the gamma-PGA biosynthesis pathway: isocitrate dehydrogenase (ICDH), glutamate dehydrogenase (GDH), and 2-oxoglutarate dehydrogenase complex (ODHC). In particular, GDH activity was increased more than 8-fold, indicating that more 2-oxoglutarate was directed to synthesis of glutamate, the substrate of gamma-PGA. Interestingly, the molecular weight of gamma-PGA remained constant regardless of CaCl2 addition. (C) 2010 Elsevier Ltd. All rights reserved.
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