期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 19, 页码 9175-9178出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.06.086
关键词
Cell biomass; Exopolysaccharide; pH-shift; pH-stat; Tremella fuciformis
资金
- Natural Science Foundation of China [30800775]
- Fundamental Research Funds for the Central Universities [27R0904083A]
The effect of pH-control modes on cell growth and exopolysaccharide production by Tremella fuciformis was evaluated in a 5-L bioreactor. The results show that the maximal dry cell weight (DCW) and exopolysaccharide production were 23.57 and 4.48 g L(-1) in pH-stat fermentation, where the maximal specific growth rate (mu(max)) and specific production rate of exopolysaccharide (P(P/X)) were 1.03 and 0.24 d(-1). respectively; under pH-shift cultivation, the maximal DCW and exopolysaccharide production were 30.57 and 3.90 g L(-1), where the mu(max) and P(P/X) were 1.21 and 0.06 d(-1). Unlike batch fermentation, maximal DCW and exopolysaccharide production merely reached 15.04 and 2.0 g L(-1), where the mu(max) and P(P/X) were 0.86 and 0.05 d(-1), respectively. These results suggest that a pH-stat strategy is a more efficient way of performing the fermentation process to increase exopolysaccharide production. Furthermore, this research has also proved that the three-stage pH-control mode is effective for cell growth. (C) 2011 Elsevier Ltd. All rights reserved.
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