期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 8, 页码 5169-5176出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.01.043
关键词
Raman spectroscopy; Glucose quantitation; Xylose quantitation; Enzymatic hydrolysate; Biofuels
资金
- U.S. Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences through the Ames Laboratory
- U.S. Department of Energy by Iowa State University [DE-AC02-07CH11358]
The effect of corn stover pretreatment on glucose quantitation in hydrolysate using Raman spectroscopy is evaluated. Dilute sulfuric-acid pretreatment results in a 20 mg mL(-1) glucose limit of detection in hydrolysate. Soaking in aqueous ammonia pretreatment produces a 4 mg mL(-1) limit of detection. Water, ethanol or hexane extraction of corn stover reduces the spectral background that limits glucose detection in dilute acid hydrolysate. Additionally. a Raman spectroscopy multi-peak fitting method is presented to simultaneously measure glucose and xylose concentration in hydrolysate. This method yields a 6.1% average relative standard error at total saccharide concentrations above 45 mg mL(-1). When only cellulase is present, glucose and xylose yield were measured by Raman spectroscopy to be 32 +/- 4 and 7.0 +/- 0.8 mg mL(-1), respectively. When both cellulase and hemicellulase were present, xylose yield increased to 18.0 +/- 0.5 mg mL(-1). Enzymatic or colorimetric assays confirmed the validity of the Raman spectroscopy results. (C) 2011 Elsevier Ltd. All rights reserved.
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