期刊
BIORESOURCE TECHNOLOGY
卷 102, 期 8, 页码 5183-5192出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.01.079
关键词
Peracetic acid; Perhydrolase; Pretreatment; Lignin; Aspen
资金
- Institute for Renewable Energy and the Environment at the University of Minnesota
- National Science Foundation [CBET-0932762]
- Ministry of Education, Science and Technology (WCU) [R32-2008-000-10213-0]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0932762] Funding Source: National Science Foundation
Release of sugars from lignocellulosic biomass is inefficient because lignin, an aromatic polymer, blocks access of enzymes to the sugar polymers. Pretreatments remove lignin and disrupt its structure, thereby enhancing sugar release. In previous work, enzymatically generated peracetic acid was used to pretreat aspen wood. This pretreatment removed 45% of the lignin and the subsequent saccharification released 97% of the sugars remaining after pretreatment. In this paper, the amount of enzyme needed is reduced tenfold using first, an improved enzyme variant that makes twice as much peracetic acid and second, a two-phase reaction to generate the peracetic acid, which allows enzyme reuse. In addition, the eight pretreatment cycles are reduced to only one by increasing the volume of peracetic acid solution and increasing the temperature to 60 degrees C and the reaction time to 6 h. For the pretreatment step, the weight ratio of peracetic acid to wood determines the amount of lignin removed. (C) 2011 Elsevier Ltd. All rights reserved.
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