期刊
MYCOBIOLOGY
卷 46, 期 3, 页码 236-241出版社
KOREAN SOC MYCOLOGY
DOI: 10.1080/12298093.2018.1513115
关键词
Non-sexual flocculation; LAMMER kinase; Lkh1; Prk1; negative regulator; Schizosaccharomyces pombe
The cation-dependent galactose-specific flocculation activity of the Schizosaccharomyces pombe null mutant of lkh1(+), the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that disruption of prk1(+), another flocculation associated regulatory kinase encoding gene, also resulted in cation-dependent galactose-specific flocculation. Deletion of prk1 increased the flocculation phenotype of the lkh1(+) null mutant and its overexpression reversed the flocculation of cells caused by lkh1 deletion. Transcript levels of prk1(+) were also decreased by lkh1(+) deletion. Cumulatively, these results indicate that Lkh1 is one of the negative regulators acting upstream of Prk1, regulating non-sexual flocculation in fission yeast.
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