期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 7, 页码 2375-2378出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.11.086
关键词
Pseudomonas stutzeri; Inorganic supports; Adsorption; Metabolic activity; Biodesulfurization
资金
- CONICYT [FONDECYT 11060018, FONDEF D0711061]
The immobilization of Pseudomonas stutzeri using adsorption on different inorganic supports was studied in relation to the number of adsorbed cells, metabolic activity and biodesulfurization (BDS). The electrophoretic migration (EM) measurements and Tetrazolioum (TTC) method were used to evaluate adsorption and metabolic activity. Results indicate that maximal immobilization was obtained with an initial load of 14 x 10(8) cells mL(-1) for Al and Sep, whereas Ti requires 20 x 10(8) cells mL(-1). The highest interaction was observed in the P. stutzeri/Si and P. stutzeri/Sep biocatalysts. The IEP values and metabolic activities indicate that P. stutzeri change the surface of supports and maintains metabolic activity. A direct relation between BDS activity and the adsorption capacity of the bacterial cells was observed at the adsorption/desorption equilibrium level. The biomodification of inorganic supports by the adsorption process increases the bioavailability of sulphur substrates for bacterial cells, improving BDS activity. (C) 2009 Elsevier Ltd. All rights reserved.
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