期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 18, 页码 7076-7082出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.03.143
关键词
Inulin; Jerusalem artichoke tubers; One-step fermentation; Paenibacillus polymyxa ZJ-9; R,R-2,3-butanediol
资金
- National Science & Technology Pillar Program [2008BAI63B07]
- Natural Science Program for Basic Research of Higher Education of Jiangsu Province [09KJB530010]
- Science and Technology Development Program of Yancheng [YK2008050]
- Natural Science Foundation of Yancheng Institute of Technology [XKY2009006]
The medium for one-step fermentation of raw inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol (R,R-2,3-BD) was developed Inulin, K2HPO4 and NH4Cl were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated according to the Box-Behnken design and determined to be 77.14 g/L, 3.09 g/L and 0.93 g/L, respectively Under the optimal conditions, the concentration of the obtained R,R-2,3-BD was 36 92 g/L, at more than 98% optical purity Compared with other investigated carbon resources, fermentation of the raw inulin extract afforded the highest yield of R,R-2,3-BD This process featured one-step fermentation of inulin without further hydrolyzing, which greatly decreased the raw material cost and thus facilitated its practical application. (C) 2010 Elsevier Ltd. All rights reserved
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