期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 6, 页码 1737-1744出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.09.092
关键词
Alkaline black liquor; Bacterial community; Seasonal dynamics; Renewable resource
资金
- Program of China
- National Programs for High Technology Research and Development
- Natural Science Foundation of China [30900043]
A microbial community which developed naturally in alkaline black liquor was investigated by culture-based and culture-independent techniques. The community was effective in lowering pH, color, and chemical oxygen demand (COD) of black liquor, and the community activities varied in different seasons. Both 16S ribosomal DNA (rDNA) clone library and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses suggested that the seasonal bacterial communities had obvious differences in diversities and compositions. Clostridium species were suggested to be the key agents in black liquor treatment. Moreover, the isolates of the genera Halomonas and Bacillus were shown to be effective in treating very heavily polluted black liquor. The strains of Halomonas, Clostridium and especially Bacillus, might be the key producers of xylanase and CMCase in the community. The worldwide problem of black liquor treatment and renewable resource utilization would benefit from these microorganisms in application potentials. (C) 2009 Elsevier Ltd. All rights reserved.
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