期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 20, 页码 7856-7863出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.04.082
关键词
Very high gravity fermentation; Corn steep liquor; Ethanol; Response surface methodology; Saccharomyces cerevisiae
资金
- Fundacao para a Ciencia e a Tecnologia (FCT), Portugal [PTDC/BIO/66151/2006, SFRH/BD/64776/2009, SFRH/BPD/44328/2008]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/44328/2008, PTDC/BIO/66151/2006, SFRH/BD/64776/2009] Funding Source: FCT
Statistical experimental designs were used to develop a medium based on corn steep liquor (CSL) and other low-cost nutrient sources for high-performance very high gravity (VHG) ethanol fermentations by Saccharomyces cerevisiae. The critical nutrients were initially selected according to a Plackett-Burman design and the optimized medium composition (44.3 g/L CSL; 2.3 g/L urea; 3.8 g/L MgSO4.7H(2)O; 0.03 g/L CuSO4.5H(2)O) for maximum ethanol production by the laboratory strain CEN.PK 113-7D was obtained by response surface methodology, based on a three-level four-factor Box-Behnken design. The optimization process resulted in significantly enhanced final ethanol titre, productivity and yeast viability in batch VHG fermentations (up to 330 g/L glucose) with CEN.PK113-7D and with industrial strain PE-2, which is used for bio-ethanol production in Brazil. Strain PE-2 was able to produce 18.6 +/- 0.5% (v/v) ethanol with a corresponding productivity of 2.4 +/- 0.1 g/L/h. This study provides valuable insights into cost-effective nutritional supplementation of industrial fuel ethanol VHG fermentations. (C) 2010 Elsevier Ltd. All rights reserved.
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