期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 20, 页码 7849-7855出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.04.076
关键词
Cellulosic ethanol; Fermentation; AFEX pretreatment; Escherichia coli; Acetate
资金
- US Department of Energy through the DOE Great Lakes Bioenergy Research Center (GLBRC) [DE-FC02-07ER64494]
The primary degradation-reaction products (DRP) identified in Ammonia Fiber Expansion (AFEX)-pretreated corn stover are acetate, lactate, 4-hydroxybenzaldehyde (4HBD) and acetamide. The effects of these products at a broad concentration range were tested on Escherichia coli KO11, a strain engineered for cellulosic ethanol production. Fermentations using glucose or xylose as the sole carbohydrate source and a sugar mixture of glucose and xylose were conducted to determine how these products and sugar selection affected fermentation performance. Co-fermentation of the sugar mixture exhibited the lowest overall ethanol productivity compared to single-sugar fermentations and was more susceptible to inhibition. Metabolic ethanol yield increased with the increasing initial concentration of acetate. Although these degradation-reaction products (with exception of acetamide) are generally perceived to be inhibitory, organic acids and 4-hydroxybenzaldehyde at low levels stimulated fermentation. Adaptation of cells to these products prior to fermentation increased overall fermentation rate. Published by Elsevier Ltd.
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