期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 16, 页码 6461-6467出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.03.064
关键词
Tetrahydrofuran; Biodegradation; Pseudomonas oleovorans; Substrate spectrum; Continuous process
资金
- National Natural Science Foundation of China [20907043]
- Zhejiang Province Graduate Innovative Scientific Research Project [YK2008033]
A Gram-negative strain DT4, capable of growing aerobically on tetrahydrofuran (THF) as the sole carbon and energy source was isolated from a pharmaceutical wastewater treatment plant. It was identified as Pseudomonas oleovorans by morphological and physiological characteristics as well as Biolog profiling and 16S rDNA sequence. Cells of P. oleovorans DT4 pre-cultured in THF could degrade 5 mM THF completely without lag phase. The generation time of 2.7 h and the maximum degradation rate of 203.9 mg THF/(h g dry weight) were observed, demonstrating that DT4 bears the highest THF-degrading activity in ever described strains. Furthermore, THF concentration as high as 100 mM was tolerated by the culture. Several important compounds including gamma-butyrrolactone and benzene could be directly metabolized, whereas other pollutants (e.g., tetrahydropyrane) could be cometabolized by DT4. THF removal was achieved in a continuous flow system with the maximum specific growth rate 0.113 h(-1) and half-saturation constant 1.224 mg/L, indicating the great potential of THF bioremediation in future full-scale application. (C) 2010 Elsevier Ltd. All rights reserved.
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