期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 4, 页码 1285-1292出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.09.044
关键词
Ammonia fiber expansion pretreatment; Forage sorghum; Sweet sorghum bagasse; Cellulosic ethanol; Fermentation
资金
- Michigan State University Research Foundation
- National Natural Science Foundation of China [20736006]
- National Basic Research Program of China [2007CB714301]
- International Collaboration Project of MOST [2006DFA62400]
- National Science and Technology Pillar Program [2007BAD42B02]
- DOE [DE-FC02-07ER64494]
With growing demand for bio-based fuels and chemicals, there has been much attention given to the performance of different feedstocks. We have optimized the ammonia fiber expansion (AFEX) pretreatment and fermentation process to convert forage and sweet sorghum bagasse to ethanol. AFEX pretreatment was optimized for forage sorghum and sweet sorghum bagasse. Supplementing xylanase with cellulase during enzymatic hydrolysis increased both glucan and xylan conversion to 90% at 1% glucan loading. High solid loading hydrolyzates from the optimized AFEX conditions were fermented using Saccharomyces cerevisiae 424A (LNH-ST) without any external nutrient supplementation or detoxification. The strain was better able to utilize xylose at pH 6.0 than at pH 4.8, but glycerol production was higher for the former pH than the latter. The maximum final ethanol concentration in the fermentation broth was 30.9 g/L (forage sorghum) and 42.3 g/L (sweet sorghum bagasse). A complete mass balance for the process is given. (C) 2009 Elsevier Ltd. All rights reserved.
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