期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 6, 页码 1820-1825出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.10.020
关键词
Hydrogen yield; Response surface methodology (RSM); Metabolic engineering; Biofuels
资金
- NSERC (the Natural Sciences and Engineering Research Council of Canada)
- Department of Foreign Affairs and International Trade, Canada DFAIT (Canada)
Metabolically engineered microbial strains can be usefully employed to give higher yields, but this also requires development of a suitable bioprocess. Maximization of product yield during fermentation requires that a number of process parameters, some of which may interact, be optimized. Here we report the effects of different fermentative process conditions; pH, temperature and glucose concentration, on the molar hydrogen yield by a genetically optimized Escherichia coli strain, DJT135. In order to simultaneously reduce the number of the experiments, and to obtain the interactions between the variables important for achieving maximum hydrogen production, a 3(K) full factorial Box-Behnken design and response surface methodology (RSM) were employed for experimental design and analysis. A maximum molar hydrogen yield of 1.69 mol H-2 mol(-1) glucose was obtained under the optimal conditions of 75 mM glucose, 35 degrees C and pH 6.5. Thus. RSM with Box-Behnken design is a useful method for achieving higher molar hydrogen yields by metabolically engineered organisms. (C) 2009 Elsevier Ltd. All rights reserved.
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