期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 8, 页码 2600-2607出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.10.059
关键词
Methanogenic inhibitor; T-RFLP; Real-time PCR; Clone library; Homoacetogenesis
资金
- Major State Basic Research Development Program (973 Program) of China [2007CB 714036]
- Tai-Lake Water Specific Program of Jiangsu Province [BS BS2007098]
- Key Technologies R & D Program (Social Development) of Jiangsu Province [BE2008627]
- State Key Lab of Urban Water Resource and Environment (HIT) [QAK200807]
- National High Technology Research and Development Program of China [2006AA06Z315]
In this study, the microbial response of anaerobic sludge digestion to the addition of two classic methanogenic inhibitors (chloroform, 2-bromoethanesulfonate) was investigated. Both the toxicants showed their effectiveness on CH4 production, whereas the hydrogen responses and acetate accumulations were elicited to different extent. Terminal restriction fragment length polymorphism analyses in combination with clone library showed that both toxicants inhibited not only methanogenic activity but the structure of methanogenic communities. The acetoclastic Methanosaetaceae was more sensitive than hydrogenotrophic Methanobacteriales and Methanomicrobiales. Interestingly, as reflected by the favorable thermodynamic condition and the increase of formyltetrahydrofolate synthetase (fhs) gene copy numbers, reductive homoacetogenesis from H-2/CO2 was also stimulated by selective inhibition of methanogenesis with 2-bromoethanesulfonate (BES). (C) 2009 Elsevier Ltd. All rights reserved.
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