期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 22, 页码 8502-8508出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.06.044
关键词
Saccharomyces cerevisiae; Asymmetric reduction; Target-oriented screening; Heterogeneous reaction system; (R)-1-phenyl-1,2-ethanediol
资金
- National Natural Science Foundation of China (NSFC) [20776060, 30800017]
- National High Technology Research and Development Program of China (863 Program) [2006AA020104, 2007AA02Z226]
- National Key Basic Research and Development Program of China (973 Program) [2009CB724706]
- Program of Introducing Talents of Discipline to Universities (111 Project) [111-2-06]
- Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) [IRT0532]
- Natural Science Foundation of Department of Science and Technology of Jiangsu province, PR China [BK2008528]
Saccharomyces cerevisiae JUC15 was successfully obtained by target reaction-oriented screening, which reduced 2-hydroxy-1-phenylethanone (HPE) to (R)-phenyl-1,2-ethanediol ((R)-PED) of excellent enantiomeric excess (e.e. >99.9%). There was no significant decrease in the yield and optical purity of (R)-PED when the free cells were reused for 40 repeated cycles at 2 g L-1 substrate concentration. The strain used cheap sucrose for cofactor regeneration and worked over a considerably wider range of pH (4-9). The optimum substrate concentration was 8 g L-1, which was higher than any other biocatalysts reported so far. Interesting, when HPE concentration reached 20 g L-1 in reaction system, where 43.2% of the substrate was present in suspended solid form, the reaction still gave enantiopure (R)-PED in 71% yield. Last but not least, the product e.e. kept above 99.9% in all examined conditions. These results suggest the potential of this strain for the industrial production of optically active (R)-PED. (C) 2010 Elsevier Ltd. All rights reserved.
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