期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 10, 页码 3649-3656出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.12.120
关键词
Pseudomonas sp M18G; Phenazine-1-carboxylic acid; Batch culture; Fed-batch culture; DO-stat feeding
资金
- National Key Basic Research Program [2009CB118906]
- National High Technology Research and Development Program of China [2006AA10A209, 2006AA02Z228, 2007AA02Z215]
- Shanghai Science and Technology Program [08391911900]
The enhancement of phenazine-1-carboxylic acid (PCA) production was investigated in a 101 fermentor using a gacA chromosomally inactivated mutant Pseudomonas sp. M18G combined with DO-stat feeing strategy. In batch culture, the optimal growth conditions with a two-stage agitation and aeration at 1.72vvm improved PCA production to 1987 mg/l at 48 h fermentation. A mathematical kinetic model consisting of cell growth, high PCA production, and substrate consumption was developed to simulate the batch culture process. Further increases in PCA production were achieved in a DO-stat fed-batch system which maintained a constant DO of 20% by a two-pulse glucose feeding strategy. With a total 6.6 g/l glucose feeding, the maximum PCA production of 2597 mg/l was obtained at 72 h fermentation, resulting in a 44.5% increase in PCA production and 10-fold greater cell numbers compared to the batch culture. The combination strategies might pave a new way for the phenazine derivative commercial production using various pseudomonads. (c) 2009 Elsevier Ltd. All rights reserved
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