期刊
BIORESOURCE TECHNOLOGY
卷 101, 期 15, 页码 6118-6123出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.02.109
关键词
Pseudomonas fluorescens BD5; Lipopeptide; Pseudofactins; Biosurfactant
资金
- Polish National Centre for Research and Development [KB/48/13639/IT1-B/U/08]
- EU [POIG.01.01.02-00-016/2008]
- Institutional Research Concept [AV0Z50200510]
The arctic freshwater bacterium Pseudomonas fluorescens BD5 produces biosurfactants when grown on 2% glucose. Crude biosurfactants were extracted from a cell-free culture supernatant with ethyl acetate and purified by preparative reversed phase high performance liquid chromatography (RP-HPLC). The chemical structure of the purified biosurfactants, pseudofactin I and II, was analyzed by matrix assisted laser desorption/ionization time of flight (MALDI TOF) mass spectrometry and tandem mass spectrometry (MS/MS). Both compounds are novel cyclic lipopeptides with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety. The C-terminal carboxylic group of the last amino acid (Val or Leu) forms a lactone with the hydroxyl of Thr3. Pseudofactin II reduced the surface tension of water from 72 mN/m to 31.5 mN/m at a concentration of 72 mg/l. Its emulsification activity and stability was greater than that of the synthetic surfactants Tween 20 and Triton X-100; pseudofactins thus have a great potential for application in industrial fields such as bioremediation or biomedicine. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据