期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 24, 页码 6543-6549出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.07.046
关键词
Sorghum bagasse; Hydrothermal pretreatment; Severity factor; Enzymatic hydrolysis; Cross-synergism
资金
- PENED 2003
- EC-European Social Fund
- Ministry of Development - General Secretariat of Research and Technology
Untreated and hydrothermally treated sorghum bagasse (SB) was hydrolyzed to simple sugars by the synergistic action of cellulases and hemicellulases produced by the fungi Fusarium oxysporum and Neurospora crassa. Synergism between the two lignocellulolytic systems was maximized with the application of higher fraction of N. crassa enzymes. Hydrothermolysis of SB was studied at a wide range of treatment times and temperatures. At intense pretreatment conditions (210 degrees C for 20 min; logR(0) = 4.54), the residual hemicellulose percentage was 17.45%, while formation of inhibitory products, 5-hydromethyl-furfural (HMF), furfural, acetic and formic acid, (0.21, 0.51, 3.36 and 1.80 g/l, respectively) remained in acceptable levels. Maximum conversion of cellulose and total polysaccharides of the untreated SB were 23.18% and 18.79%, respectively. Combining hydrothermal treatment and enzymatic hydrolysis of released oligosaccharides and insoluble solids resulted in improvement of cellulose (approximately 15% increase) and total polysaccharides (two fold) hydrolysis compared to that of untreated SB. (C) 2009 Elsevier Ltd. All rights reserved.
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