期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 21, 页码 5170-5175出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.05.049
关键词
Enzymatic hydrolysis; Echinodontium taxodii; Biological pretreatment; Adsorption; Wood
资金
- Foundation of China's National Grand Fundamental [2007CB210200]
Selective white-rot fungi have shown potential for lignocellulose pretreatment. In the study, a new fungal isolate, Echinodontium taxodii 2538, was used in biological pretreatment to enhance the enzymatic hydrolysis of two native woods: Chinese willow (hardwood) and China-fir (softwood). E taxodii preferentially degraded the lignin during the pretreatment, and the pretreated woods showed significant increases in enzymatic hydrolysis ratios (4.7-fold for hardwood and 63-fold for softwood). To better understand effects of biological pretreatment on enzymatic hydrolysis, enzyme-substrate interactions were investigated. It was observed that E. taxodii enhanced initial adsorption of cellulase but which did not always translate to high initial hydrolysis rate. However, the rate of change in hydrolysis rate declined dramatically with decreasing irreversible adsorption of cellulase. Thus, the enhancement of enzymatic hydrolysis was attributed to the decline of irreversible adsorption which may result from partial lignin degradation and alteration in lignin structure after biological pretreatment. (C) 2009 Elsevier Ltd. All rights reserved.
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