期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 24, 页码 6378-6384出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.07.049
关键词
Laccase; Phenols; Steam-explosion; Bioethanol; Wheat straw
资金
- CENIT [AGL-2007-66416-C05-02]
In this work we compared the efficiency of a laccase treatment performed on steam-exploded wheat straw pretreated under soft conditions (water impregnation) or harsh conditions (impregnation with diluted acid). The effect of several enzymatic treatment parameters (pH, time of incubation, laccase origin and loading) was analysed. The results obtained indicated that severity conditions applied during steam explosion have an influence on the efficiency of detoxification. A reduction of the toxic effect of phenolic compounds by laccase polymerization of free phenols was demonstrated. Laccase treatment of steam-exploded wheat straw reduced sugar recovery after enzymatic hydrolysis, and it should be better performed after hydrolysis with cellulases. The fermentability of hydrolysates was greatly improved by the laccase treatment in all the samples. Our results demonstrate the action of phenolic compounds as fermentation inhibitors, and the advantages of a laccase treatment to increase the ethanol production from steam-exploded wheat straw. (c) 2009 Elsevier Ltd. All rights reserved
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