期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 5, 页码 1852-1859出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2008.10.005
关键词
Ganoderma lucidum; pH-shift culture; DOT-shift culture; Fed-batch culture; Integrated strategy
资金
- National Natural Science Foundation of China [20706012]
- National High Technology Research and Development Key Program of China [2007AA021506]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars (Ministry of Personnel, State Education Ministry and Hubei Provincial Department of Personnel)
- Hubei Provincial Innovative Research Team in University [T200608]
- Hubei Provincial Science Foundation for Distinguished Young Scholars [2006ABB034]
- Hubei Provincial International Cooperation Foundation for Scientific Research [2007CA012]
- Science and Technology Commission of Wuhan Municipality [20065004116-31]
- Scientific Research Key Foundation from Hubei University of Technology [306.18002]
- Open Project Program of the State Key Laboratory of Bioreactor Engineering (ECUST)
- Hubei Provincial Department of Education, China
Investigations on Ganoderma lucidum fermentation suggested that the responses of the cell growth and metabolites biosynthesis to pH and dissolved oxygen tension (DOT) were different. The ganoderic acid (GA) production of 321.6 mg/L was obtained in the pH-shift culture by combining a 4-day culture at pH 3.0 with the following 6-day culture at pH 4.5, which was higher by 45% and 300% compared with the culture at pH 3.0 and 4.5, respectively. The CA production of 487.1 mg/L was achieved in the DOT-shift culture by combining a 6-day culture at 25% of DOT with a following 6-day culture at 10% of DOT, which was higher by 43% and 230% compared with the culture at 25% and 10% of DOT, respectively. A fed-batch fermentation process by combining the above-mentioned pH-shift and DOT-shift strategies resulted in a significant synergistic enhancement of CA accumulation up to 754.6 mg/L, which is the highest reported in the submerged fermentation of G. lucidum in stirred-tank bioreactor. (C) 2008 Elsevier Ltd. All rights reserved.
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