期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 13, 页码 3410-3414出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.02.031
关键词
2,3-Butanediol; Klebsiella oxytoca; Batch fermentation; Two-stage control strategy
资金
- National Natural Science Foundation of China [20606018, 30800028]
- National Basic Research Program of China [2007CB707805]
- National High Technology Research and Development Program of China [2006AA02Z244]
- Post-doctoral Science Fund of Jiangsu Province [0701035B]
- Innovation Fund for Doctoral Dissertation of Nanjing University of Technology [BSCX200808]
Batch fermentative production of 2,3-butanediol by Klebsiella oxytoca was investigated using various oxygen supply methods though varying agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (mu). specific glucose consumption rate (q(s)) and specific 2,3-butanediol formation rate (q(p)), a two-stage agitation speed control strategy, aimed at achieving high concentration, high yield and high productivity of 2.3-butanediol, was proposed. At the first 15 h, agitation speed was controlled at 300 rpm to obtain high mu for cell growth, subsequently agitation speed was controlled at 200 rpm to maintain high q(p) for high 2.3-butanediol accumulation. Finally, the maximum concentration of 2,3-butanediol reached 95.5 g l(-1) with the yield of 0.478 g g(-1) and the productivity of 1.71 g l(-1) h(-1), which were 6.23%, 6.22% and 22.14% over the best results controlled by constant agitation speeds. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
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