期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 2, 页码 1015-1019出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2008.07.015
关键词
Biosurfactant; Marine Bacillus circulans; Carbon substrates; HPTLC analysis; RP-HPLC
资金
- Indian Institute of Technology, Kharagpur
- Council of Scientific and Industrial Research, New Delhi
- Department of Biotechnology, Government of India [BT/PR-6827/AAQ/03/263/2005]
The potential of a marine microorganism to utilize different carbon substrates for the production of an extracellular biosurfactant was evaluated. Among the several carbon substrates tested for this purpose, production of the crude biosurfactant was found to be highest with glycerol (2.9 +/- 0.11 g L-1) followed by starch (2.5 +/- 0.11 g L-1), glucose (1.16 +/- 0.11 g L-1) and sucrose (0.94 +/- 0.07 g L-1). The crude biosurfactant obtained from glycerol, starch and sucrose media had significantly higher antimicrobial action than those obtained from glucose containing medium. RP-HPLC resolved the crude biosurfactants into several fractions one of which had significant antimicrobial action. The antimicrobial fraction was found in higher concentrations in biosurfactant obtained using glycerol, starch and sucrose as compared to the biosurfactants from glucose medium, thereby explaining higher antimicrobial activity. The carbon substrate was thus found to affect biosurfactant production both in a qualitative and quantitative manner. (c) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据