期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 3, 页码 1192-1196出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2008.07.070
关键词
Hydrogen production; Ethanol fermentation; Response surface methodology; Ethanoligenens harbinense B49; Culture conditions
资金
- National Natural Science Fund of China [30470054]
- National Natural Science Key Fund of China [50638020]
The design of an optimum and cost-efficient medium for high-level production of hydrogen by Ethanoligenens harbinense B49 was attempted by using response surface methodology (RSM). Based on the Plackett-Burman design, Fe2+ and Mg2+ were selected as the most critical nutrient salts. Subsequently, the optimum combination of the selected factors and the sole carbon source glucose were investigated by the Box-Behnken design. Results showed that the maximum hydrogen yield of 2.21 mol/mol glucose was predicted when the concentrations of glucose, Fe2+ and Mg2+, were 14.57 g/L, 177.28 mg/L and 691.98 mg/L, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective glucose, Fe2+ and Mg2+ concentration of 14.5 g/L, 180 mg/L and 690 mg/L, the initial pH of 6.0 and experimental temperature of 35 +/- 1 degrees C. Without further pH adjustment, the maximum hydrogen yield of 2.20 mol/mol glucose was obtained based on the optimized medium with further verified the practicability of this optimum strategy. (c) 2008 Elsevier Ltd. All rights reserved.
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