期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 2, 页码 826-832出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2008.06.047
关键词
Poly(gamma-glutamic acid); Bacillus licheniformis; Optimization; Plackett-Burman design; Response surface methodology
This paper reports on the optimization of poly(gamma-glutamic acid) (PGA) production by Bacillus licheniformis NCIM 2324 using a statistical approach. One-factor-at-a-time method was used to investigate the effect of carbon sources, nitrogen sources and pH on PGA production. Plackett-Burman design was adopted to select the most important nutrients influencing the yield of PGA. After identifying effective nutrients, response surface methodology was used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher PGA production, and confirm its validity experimentally. PGA production increased significantly from 5.27 to 26.12 g/l when the strain was cultivated in the optimal medium developed by using statistical approach, as compared to basal medium. (C) 2008 Elsevier Ltd. All rights reserved.
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