期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 2, 页码 896-902出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2008.07.009
关键词
Esterases; Solvent tolerance; B. licheniformis; Stress; Non-aqueous biocatalysis
资金
- CONICET [PIP 6203/06]
- ANPCyT [PICT 14-32491]
A thermophile, halotolerant and organic-solvent-tolerant esterase producer Bacillus sp. S-86 strain previously isolated was found to belong to Bacillus licheniformis species through morphological, biochemical, 16S rRNA gene sequence analyses and rDNA intergenic spacers amplification (ITS-PCR). The strain can grow at 55 degrees C in presence of C2-C7 alkanols (log P = -0.86 to 2.39), and NaCl concentrations up to 15% (w/v). This bacterium showed optimal growth and esterase production at 50 degrees C. Two different molecular weight esterase activities were detected in zymographic assays. PMSF inhibited type I esterase activity, showing no inhibitory effect on type 11 esterase activity. B. licheniformis S-86 was able to grow in presence of hydroxylic organic-solvents like propan-2-ol, butan-1-ol and 3-methylbutan-1-ol. At a sub-lethal concentration of these solvents (392 mmol l(-1) propan-2-ol; 99 mmol l(-1) butan-1-ol, 37 mmol l(-1) 3-methylbutan-1-ol), adequate to produce 50% cell growth inhibition at 50 degrees C, an increment between 1.9 and 2.3 times was observed in type I esterase production, and between 2.2 and 3.1 times in type 11 esterase production. (C) 2008 Elsevier Ltd. All rights reserved.
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