期刊
BIORESOURCE TECHNOLOGY
卷 100, 期 24, 页码 6665-6668出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.07.029
关键词
Maillard product; Degradation; Alcaligenes faecalis; Bacillus cereus; Metabolite characterization
资金
- University Grants Commission
- CSIR [NWP-19]
Two aerobic bacteria RNBS1 and RNBS3 capable to degrade and utilize sucrose-glutamic acid Maillard products (SGMPs) as carbon, nitrogen and energy source were isolated and characterized as Alcaligenes faecalis (DQ659619) and Bacillus cereus (DQ659620) respectively by 16S rRNA gene sequence analysis. In present study, mixed bacterial culture was found more effective compared to axenic culture RNBS1 and RNB53 decolourizing 73.79%, 66.80% and 62.56% SGMPs, respectively. The SGMPs catabolizing enzyme was characterized as manganese dependent peroxidase (MnP) by SDS-PAGE yielding a single band of 43 KDa. Further, the LC-MS-MS and other spectrophotometric analysis have revealed that most of the SGMPs detected in control were diminished from bacteria treated samples. The disappearance of SGMPs from bacteria treated samples could be related with the degradation of SGMPs. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据