期刊
BIORESOURCE TECHNOLOGY
卷 99, 期 6, 页码 1530-1537出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2007.04.031
关键词
solid-state fermentation; response surface methodology; Metarhizium anisopliae; conidiospores
Optimization of conidial production was achieved by response surface methodology (RSM), a powerful mathematical approach widely applied in the optimization of fermentation process, using the three substrates; rice, barley and sorghum at variable pH, moisture content and yeast extract concentrations. These three factors were found to be important, affecting Metarhizium anisopliae spore production. A 2 3 full factorial central composite design and RSM were applied to determine the optimal concentration of each variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. Moisture content of 75.68% for sorghum, 73.21% for barley and 22.34% for rice produced optimal results. Maximal conidial yield was recorded for rice at a pH of 7.01; at 7.06 for sorghum and at 6.76 for barley. (C) 2007 Published by Elsevier Ltd.
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