期刊
BIORESOURCE TECHNOLOGY
卷 99, 期 11, 页码 4603-4608出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2007.07.030
关键词
biosurfactant; Bacillus subtilis; surface tension; interfacial tension; MEOR; biocontrol agent
A biosurfactant producing strain, Bacillus subtilis 20B, was isolated from fermented food in India. The strain also showed inhibition of various fungi in in-vitro experiments on Potato Dextrose Agar medium. It was capable of growth at temperature 55 degrees C and salts up to 7%. It utilized different sugars, alcohols, hydrocarbons and oil as a carbon source, with preference for sugars. In glucose based minimal medium it produced biosurfactant which reduced surface tension to 29.5 mN/m, interfacial tension to 4.5 mN/m and gave stable emulsion with crude oil and n-hexadecane. The biosurfactant activity was stable at high temperature, a wide range of pH and salt concentrations for five days. Oil displacement experiments using biosurfactant containing broth in sand pack columns with crude oil showed 30.22% recovery. The possible application of organism as biocontrol agent and use of biosurfactant in microbial enhanced oil recovery (MEOR) is discussed. (C) 2007 Elsevier Ltd. All rights reserved.
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